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Image Search Results
Journal: Frontiers in Oncology
Article Title: Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models
doi: 10.3389/fonc.2022.818437
Figure Lengend Snippet: List of IMC antibodies.
Article Snippet: 165 Ho ,
Techniques:
Journal: Frontiers in oncology
Article Title: Wnt/β-Catenin Inhibition Disrupts Carboplatin Resistance in Isogenic Models of Triple-Negative Breast Cancer.
doi: 10.3389/fonc.2021.705384
Figure Lengend Snippet: FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform DN90 and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).
Article Snippet: For overexpression of DN90-b-catenin, we used
Techniques: Over Expression, Gene Expression, Western Blot, Transduction, Plasmid Preparation, Microscopy, One-tailed Test, Cytometry, Staining, Activity Assay, Immunolabeling, Generated