β catenin Search Results


94
Bioss anti β catenin
Anti β Catenin, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological β catenin protein
β Catenin Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc antibody b catenin cat 9587s cell signaling
Antibody B Catenin Cat 9587s Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
fluidigm beta catenin
List of IMC antibodies.
Beta Catenin, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc active β catenin
List of IMC antibodies.
Active β Catenin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Addgene inc emerald β catenin
List of IMC antibodies.
Emerald β Catenin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Addgene inc plv betacatenin dn90
FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform <t>DN90</t> and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).
Plv Betacatenin Dn90, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Addgene inc lentiviral plasmid encoding β catenin
FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform <t>DN90</t> and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).
Lentiviral Plasmid Encoding β Catenin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Addgene inc pt3 ctnnb1 n90 vector
FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform <t>DN90</t> and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).
Pt3 Ctnnb1 N90 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Addgene inc pcdna3 δn47 β catenin
FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform <t>DN90</t> and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).
Pcdna3 δn47 β Catenin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Addgene inc β catenin
FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform <t>DN90</t> and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).
β Catenin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pcdna3 s33y β catenin
FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform <t>DN90</t> and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).
Pcdna3 S33y β Catenin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


List of IMC antibodies.

Journal: Frontiers in Oncology

Article Title: Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models

doi: 10.3389/fonc.2022.818437

Figure Lengend Snippet: List of IMC antibodies.

Article Snippet: 165 Ho , beta-catenin §§ , D13A1 , 3165032D , Fluidigm , 1:150.

Techniques:

FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform DN90 and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).

Journal: Frontiers in oncology

Article Title: Wnt/β-Catenin Inhibition Disrupts Carboplatin Resistance in Isogenic Models of Triple-Negative Breast Cancer.

doi: 10.3389/fonc.2021.705384

Figure Lengend Snippet: FIGURE 3 | b-catenin overexpression in 468’P induces carboplatin-resistance, pluripotency-related gene expression, and cancer stem cell features. (A) Western blot (top) of total b-catenin in MDA-MB-468 cells transduced with an empty vector or truncated, constitutively active b-catenin isoform DN90 and phase-contrast microscopy (down). (B) Enriched gene sets from Wikipathways database by one-tailed GSEA of ranked DEGs between 468’OE and 468’P sorted by normalized enrichment score (left) and enrichment map illustrating pathways most significantly different between 468’OE and 468’P (right). (C) Non-linear fit model of [CAR] vs. normalized response for IC50 determination. (468’OE: n=6, R2 = 0.92; 468’CTRL: n=6, R2 = 0.95). (D) Representative flow cytometry scatterplots of annexin V staining (left) of 468’CTRL and 468’OE cells treated with carboplatin 2 mM for 72h and statistical analysis of the mean frequency of annexin V positive cells using one-way ANOVA corrected for multiple comparisons using the Holm-Sidak method (right, n=3). (E) mRNA level fold change (Log2) of CTNNB1 (b-catenin), Wnt target AXIN2, and stem cell markers in 468’OE cells vs. 468’CTRL (n=4). Multiple t-tests with Holms-Sidak correction for multiple comparisons. (F) Representative scatterplots of flow cytometric analysis of aldehyde dehydrogenase activity (left) and statistical analysis of the mean percentage of ALDH+ cells in 468’OE (n=5) and 468’CTRL (n=5) using Welch’s t-test (right). (G) Representative scatterplots of flow cytometric analysis of CD44-PE and CD24-APC immunolabeling (left) and corresponding statistical analysis of the mean percentage of CD44+/CD24- cells using Welch’s t-test (right; n=3). (H) Representative brightfield images of tumorspheres generated from 468’CTRL and 468’OE cells (left, scale bar: 50 mm) and statistical analysis of mean tumorsphere forming units (number of spheres/number of seeded single cells) using Welch’s t-test (right; n=3). (Barplots represent mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non significant).

Article Snippet: For overexpression of DN90-b-catenin, we used pLV-betacatenin DN90 (Addgene, #36985) and pPRIME-CMV-NEOrecipient (CTRL, Addgene, #11659).

Techniques: Over Expression, Gene Expression, Western Blot, Transduction, Plasmid Preparation, Microscopy, One-tailed Test, Cytometry, Staining, Activity Assay, Immunolabeling, Generated